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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 12 Documents
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Search results for , issue "Vol 6, No 2 (2016)" : 12 Documents clear
Bioactivity of Sweet Flag (Acorus calamus Linnaeus) Essential Oils against Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Melani, Dewi; Himawan, Toto; Afandhi, Aminudin
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.04

Abstract

The study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a com-pletely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and anti-feedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatog-raphy-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone. Keywords:  Acorus calamus, essential oils, mortality,  antifeedant,  Spodoptera lituraThe study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a completely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and antifeedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatography-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone.
PCR-RFLP and Sequencing of trnS/trnfM Fragment of Enhalus acoriodes from Sanur Coastal Waters, Bali, Indonesia: A Preliminary Study Pharmawati, Made; Imaniar, Eka Fibayani
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.10

Abstract

This study aimed to evaluate the resolution of PCR-RFLP and sequencing of trnS/trnfM in detecting genetic diversity of seagrass Enhalus acoroides at Sanur Beach, Sindhu Beach and Semawang Beach. This research used six samples E. acoroides from each location. DNA extraction method followed Doyle and Doyle. PCR amplifications were done using primers P672/P673, P674/P675 and ITS4/ITS5 followed by digestion using restriction enzymes EcoRI, PstI, HindIII, BamHI, Rsa, Mva and HinfI. Sequencing analysis used PCR products of primers trnS/trnfM. Testing of DNA sequences of E. acoroides were conducted using BLAST (Basic Local Alignment Search Tool). DNA sequences were further analyzed using MEGA 5.2 software (Molecular Evolutionary Genetic Analysis) to evaluate variations of DNA. The sequence alignments were done using ClustalW software to determine the homology between the DNA sequences. The results showed that 18 samples of E. acoroides from Sanur, Sindhu and Semawang Beach have no polymorphism based on restriction enzyme analyses. Furthermore, sequencing of trnS/trnfM region of 18 samples E. acoroides showed that the sequences were identical.   
Effects of Compost on Nematode Pratylenchus sp. (Tylenchida: Pratylenchidae) Population in Patchouli Subarjah, Cecep; Himawan, Toto; Puspitarini, Retno Dyah
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.07

Abstract

Patchouli is seen as one of the most important export commodities in Indonesia. The presence of parasitic nematode Pratylenchus sp. creates a barrier to plant cultivation. Increasing organic content in soil by applying compost in the plantation is one of approaches in controlling the nematode. This research was mainly aimed to study the effects of compost fertilization on the population growth of nematode Pratylenchus sp. and yields on patchouli. These research used a Completely Randomised Design (CRD) consisting of 12 treatments with three replicates. Selected plant was inoculated with nematodes with the following initial population: 100, 200, 300, 400, and 500 nematodes in soil with and without compost. The plant with no inoculation was used as control. The result of the research confirmed that the addition of compost was capable of suppressing the population of nematode Pratylenchus sp. as much as 58.93 - 67.31% and 40.74 - 59.19% in root and in soil of each treatment, respectively. Compost applied in each treatment positively affected the increasing amount of Patchouli oil yielded, and it could also reduce the loss as much as 18.34 - 43.36%. Trichoderma sp. was found to be dominant in compost in the treatment with its population of 2.1 × 104 propogul/g.
Litter Decomposition Rate of Avicennia marina and Rhizophora apiculata in Pulau Dua Nature Reserve, Banten Siska, Febriana; Sulistijorini, Sulistijorini; Kusmana, Cecep
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Litter decomposition rate is useful method to determine forest fertility level. The aims of this study were to measure decomposition rate, and analyze the nutrient content released organic carbon, nitrogen, and phosphor from Avicennia marina and Rhizophora apiculata litters during the decomposition process. The research was conducted in the Pulau Dua Nature Reserve, Serang-Banten on A. marina and R. apiculata forest communities. Litter decomposition rate measurements performed in the field. Litter that has been obtained with the trap system is inserted into litter bag and than tied to the roots or trees to avoid drifting sea water. Litter decomposition rate was measured every 15 days and is accompanied by analysis of the content of organic C , total N and P. Our research results showed decomposition rate of A. marina (k= 0.83) was higher than that of R. apiculata (k= 0.41). Differences of  leaf anatomical structure and sea water salinity  influenced to the rate of litter decomposition. Organic C released was declined with longer of litter decomposition, on the contrary of releasing N and P nutrients.
Human and Animal Pentastomiasis in Malaysia : Review Latif, Bahaa Mohamed Abdul; Muslim, Azdayanti; Chin, Heo Chong
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.12

Abstract

Pentastomiasis is a zoonotic parasitic disease induced by the larval stages of pentasomes. The disease has been reported in Africa, the Middle East and Southeast Asia and caused by the nymphs of the two genera:  Linguatula  and Armillifer and the two species L. serrata and A. armillatus regard for more than 90% of human cases. The definitive hosts of Armillifer spp. are snakes, lizards and other reptiles. The parasites live in the upper respiratory tracts and lay eggs that are passed out through respiratory secretions, saliva or faeces. Intermediate hosts are  humans, rodents and other mammals. Humans incidentally acquire the infestation by the consumption of uncooked infested snake meat or by drinking water contaminated with ova of the pentastomes. In the intestinal tract, the larvae hatch from the ova, penetrate the intestinal wall and migrate to organs in which the liver is the most common site. Human pentastomiasis was reported among aborigines in West and East Malaysia. Armillifer moniliformis was identified  in wild animals and carnivores with infection rate 1.8% and 20.7% respectively. The adults of pentostomes (A. moniliformis) were recovered from two out of six snakes Python reticulates. Recently a case of human pentastomiasis was reported in Sabah, East Malaysia, caused by nymph of Armillifer moniliformis.
Botanical Exploration and Crater Vegetation Survey of Mt. Galunggung, West Java Zuhri, Musyarofah; Wiriadinata, Harry; Astuti, Ratna Suti; Hadiwaluyo, Supan; Syamsudin, Syamsudin
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.02

Abstract

Botanical exploration at Mt. Galunggung protected forest, West Java was conducted in June 2014. This study was aimed to collect plant living specimen for ex-situ conservation purpose of Kuningan Botanic Garden and to conduct vegetation analysis in crater area. Random inventory method at 600-1200 m asl was used to collect plant in that area. Vegetation analysis of tree, shrub and herb were used line transect methods. Result showed that 273 collection numbers of plant living species were collected from Mt. Galunggung, which consisted of 213 species, 143 genus and 72 families. Plant families which most collected were Moraceae, Euphorbiaceae, Arecaceae and Lauraceae respectively. In crater area was collected a lot of pioneer plant, such as dawola (Trema cannabina Lour.), paku tiang (Cyathea spp.), nangsi (Villebrunea rubescens (Blume) Blume), muncang cina (Ostodes paniculata Blume), mara (Macaranga tanarius (L.) Mull.Arg.), and kareumbi (Homalanthus populneus (Geiseler) Pax). Furthermore, in secondary remnant forest were collected typical plants of Java mountains such as saninten (Castanopsis argentea (Blume) A.DC., puspa (Schima wallichii Choisy) and some of tree seedlings from Lauraceae family. Vegetation affected by eruption in Mt. Galunggung is in early stage of forest succession and still develops to next stage of succession. Tree, shrub and herb in the crater area were dominated by Cyathea contaminans (Wall. Ex Hook.) Copel., Calliandra callothyrsus Meisn. and Ageratina riparia (Regel) R.M.King & H.Rob. with H’ was 0.35, 0.31 and 0.30 respectively.
Diversity of Macroalgae in Kasiak Gadang Island Nirwana Beach, Padang - West Sumatra, Indonesia Hadi, Faisal; Zakaria, Indra Junaidi; Syam, Zuhri
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.06

Abstract

This study had been conducted in October – November 2014. This study aims to know about the diversity of macroalgae in Kasiak Gadang Island, Nirwana Beach, Padang, West Sumatra Indonesia. The method used in this research is line and belt transect. The result of this study is five species of macroalgae founded on this island. There are Padine minor, Sargassum crassifolium, Sargassum cristaefolium, Turbinaria decurrens, and Halimeda sp. Based on relative density, P. minor had 67.15% at Station I and 51.72% at Station II. S. crassifolium had 83.52% at Station III. T. decurrens had 50.86% at Station IV. The index of diversity of macroalgae in Kasiak Gadang Island is about 0.48 – 1.27. According to the diversity index criteria, macroalgae diversity in this island is considered being in low.
Improvement of Trimethylamine Uptake by Euphorbia milii: Effect of Inoculated Bacteria Siswanto, Dian; Thiravetyan, Paitip
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.11

Abstract

In the last few years, a great emphasis has been placed on phytoremediation of indoor air pollution studies. However, limited work has been addressed to observe the bacteria potential to assist the phytoremediation process of trimethylamine (TMA). In this work, the ability of 4 different bacteria for individual TMA removal and IAA production were observed. In addition, the enhancement of TMA removal efficiency by Euphorbia milii with various inoculating bacteria were investigated. Bacillus thuringiensis, Citrobacter amalonaticus Y19, Bacillus nealsonii, and white colony-soil bacteria (WCSB) were able to absorb TMA and produce IAA individually. B. thuringiensis and C. amalonaticus Y19 were the two most effective bacteria to improve TMA removal efficiency by the plant. Since concentrations of IAA production by individual bacterium were highly correlated with TMA removal efficiency by plants in early periods of fumigation and highly correlated with leaf IAA production of bacterially inoculated plants, two predicted mechanisms on improving TMA uptake by bacterially inoculated plants are presented: (1) bacteria migration from plant roots to leaves increases leaf IAA concentration and (2) increasing concentration of bacterially inoculated root IAA inhibits transportation of IAA from leaves to roots, resulting in higher leaf IAA concentration. The higher concentration of leaf IAA is suggested to be a factor to increase stomatal opening which improves TMA removal efficiency of the plant.
Kinetic Parameters Analysis of Liver and Kidney Catalase Under The Influence of Cadmium and Mercury In Vitro Karantika, Ellsa Anggun; Supianur, Supianur; Edyson, Edyson; Suhartono, Eko
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.01

Abstract

Cadmium (Cd) and mercury (Hg) are toxic metals that affect human organs function, including liver and kidney. This toxic activity is because the heavy metal could induce oxidative stress and interfere antioxidant activities, including catalase (CAT). The present study was aims to evaluate the effect of Cd and Hg to liver and kidney CAT kinetic parameters in vitro. In this experiment, liver and kidney were taken from male rats (Rattus novergicus). Sample the homogenized and divided into three groups with; T0 served as control which contains liver or kidney homogenate + H2O2, T1 which contains liver or kidney homogenate + H2O2 + 0.03 mg/L CdSO4; and T2 which contains liver or kidney homogenate + H2O2 + 1 mg/L Hg. Solutions then incubated at 37ºC for 1 hour and then was prepared for CAT activity measurement. The CAT activity was measured using spectrophotometer at 240 nm. For measuring the kinetic parameters, different concentration of H2O2 were used. The kinetics parameters (Km and Vmax) were calculated using Lineweaver-Burk plot. The results shows that Cd and Hg could decrease the affinity of CAT-H2O2 complex which expressed by the higher Km and Vmax values. Also from the results, Cd has better activity to decreased the affinity of CAT-H2O2 complex than Hg. From this results, it can be concluded that Cd and Hg treatments could inhibit CAT activity in liver and kidney in vitro.
Litter Decomposition Rate of Karst Ecosystem at Gunung Cibodas, Ciampea Bogor Indonesia Sari, Sethyo Vieni; Qayim, Ibnul; Hilwan, Iwan
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.08

Abstract

The study aims to know the productivity of litter and litter decomposition rate in karst ecosystem. This study was conducted on three altitude of 200 meter above sea level (masl), 250 masl and 300 masl in karst ecosystem at Gunung Cibodas, Ciampea, Bogor. Litter productivity measurement performed using litter-trap method and litter-bag method was used to know the rate of decomposition. Litter productivity measurement results showed that the highest total of litter productivity measurement results was on altitude of 200 masl (90.452 tons/ha/year) and the lowest was on altitude of 300 masl (25.440 tons/ha/year). The litter productivity of leaves (81.425 ton/ha/year) showed the highest result than twigs (16.839 ton/ha/year), as well as flowers and fruits (27.839 ton/ha/year). The rate of decomposition was influenced by rainfall. The decomposition rate and the decrease of litter dry weight on altitude of 250 masl was faster than on the altitude of 200 masl and 300 masl. The dry weight was positively correlated to the rate of decomposition. The lower of dry weight would affect the rate of decomposition become slower. The average of litter C/N ratio were ranged from 28.024%--28.716% and categorized as moderate (>25). The finding indicate that the rate of decomposition in karst ecosystem at Gunung Cibodas was slow and based on C/N ratio of litter showed the mineralization process was also slow.

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